Identification of new aphid vector species of Blueberry scorch virus

D. Thomas Lowery, Michael G. Bernardy, Robyn M. Deyoung, Chris J. French

Abstract


Transmission of Blueberry scorch virus (BlScV) by the aphid species Ericaphis fimbriata (Richards), Aphis spiraecola (Patch), Aphis pomi DeGeer, Acyrthosiphon pisum (Harris), Myzus ornatus Laing, Aphis helianthi Monell, Myzus persicae (Sulzer), and Rhopalosiphum padi (L.), was studied in the laboratory using timed aphid acquisition feeding periods and known numbers of aphid vectors. Successful infection of Nicotiana occidentalis Wheeler (Solanaceae), a newly identified herbaceous host, and highbush blueberry, Vaccinium corymbosum L. (Ericaceae), following brief virus-acquisition feeds lasting less than 5 min, demonstrated that BlScV was transmitted in a nonpersistent, non-circulative manner. Based on transfer of 10 aphids per plant, the most efficient vector of BlScV from infected to healthy N. occidentalis was M. ornatus. Compared with this herbaceous host, infection rates for blueberry were much lower even though higher numbers of aphids (25/plant) were used. The highest rate of infection for blueberry (20%) was achieved when the green colour form of E. fimbriata was used to transmit the virus. The relatively low rate of transmission from infected to healthy blueberry suggests that BlScV would spread slowly in the field. Planting of certified virus-free nursery material and aggressive removal of infected plants should help control this economically important disease of highbush blueberries.

Keywords


Blueberry scorch virus; aphid vectors; virus transmission

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References


Bernardy, M.G., C.R. Dubeau, A. Braun, C.E. Harlton, A. Bunckle, L.A. Wegener, D.T. Lowery and C.J. French. 2005. Molecular characterization and phylogenetic analysis of two distinct strains of blueberry scorch virus from westem Canada. Canadian Journal of Plant Pathology 27: 581-591.

Bristow, P.R., R.R. Martin and G.E. Windom. 2000. Transmission, field spread, cultivar response, and impact on yield in highbush blueberry infected with blueberry scorch virus. Phytopathology 90: 474-479.

Caruso, F.L. and D.C. Ramsdell. 1995. Compendium of Blueberry and Cranberry Diseases. APS Press, St. Paul, MN.

Catlin, N.J. and S.G. Schloemann. 2004. Blueberry Scorch Virus (BlScV). UMass Amherst Extension Factsheet, F-129-2004, University of Massachusetts, Amherst, MA.

Cavileer, T.D., B.T. Halpern, D.M. Lawrence, E.V. Podleckis, R.R. Martin and B.I. Hillman. 1994. Nucleotide sequence of the carlavirus associated with blueberry scorch and similar diseases. Journal of General Virology 75: 711-720.

Ciuffo, M., D. Pettiti, S. Gallo, V. Masenga and M. Turina. 2005. First report of blueberry scorch virus in Europe. Plant Pathology 54: 565.

Clark, M.F. and A.N. Adams. 1977. Characteristics of the n1icroplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34: 475-483.

Foster, G.D. 1992. The structure and expression of the genome of carlaviruses. Research Virology 143: 103-112.

Harris, K.F. 1983. Sternorrhynchous vectors of plant viruses: virus-vector interactions and transmission mechanisms. Advances in Virus Research 28: 113-140.

Hillman, B.I., D.M. Lawrence and B.T. Halpem. 1995. Characterization and detection of blueberry scorch carlavirus and red ringspot virus caulimovirus. Journal of Small Fruit and Viticulture 3: 83-93.

Lowery, D.T. 1997 . Turnip Mosaic Virus Monitoring and Research Program Final Report. Ontario Ministry of Agriculture, Food, and Rural Affairs, Guelph, ON.

Lowery, D.T., K.C. Eastwell and M.J. Sn1irle. 1997. Neem seed oil inhibits transmission of Potato virus Y to pepper. Annals of Applied Virology 130: 217-225.

Lowery D.T., C.J. French and M.G. Berr1ardy. 2005. Nicotiana occidentalis: a new herbaceous host for blueberry scorch virus. Plant Disease 89: 205.

Maramorosch, K. 1963. Arthropod transmission of plant viruses. Annual Review of Entomology 8: 369414.

Martin, R.R. and P.R. Bristow. 1988. A carlavirus associated with blueberry scorch disease. Phytopathology 78: 1636-1640.

Ng, C.K. and K.L. Perry. 2004. Transmission of plant viruses by aphid vectors. Molecular Plant Pathology 5: 505-511.

Podleckis, E.V. and R.F. Davis. 1989. Infection of highbush blueberries with a putative carlavirus. Acta Horticulturae 241: 338—343.

Raccah, B. 1986. Nonpersistent viruses: epidemiology and control. Advances in Virus Research. 31: 387429.

Raworth, D.A., C.J. French, D.T. Lowery, M.G. Bernardy, M. Bouthillier, S. Mathur, C.-K. Chan, R.G. Foottit, E. Maw, L.A. Wegener and M. Sweeney. 2008. Temporal trends in the transmission of Blueberry scorch virus in British Columbia, Canada. Canadian Journal of Plant Pathology 30: 345-350.

Raworth, D.A., C.-K. Chan, R.G. Foottit and E. Maw. 2006. Spatial and temporal distribution of winged aphids (Hemiptera: Aphididae) frequenting blueberry fields in southwestern British Cohnnbia and implications for the spread of Blueberry scorch virus. The Canadian Entomologist 138: 104-113.

Wegener, L.A., R.R. Martin, M.G. Bernardy, L. MacDonald and Z.K. Punja. 2006. Epidemiology and identification of strains of blueberry scorch virus on highbush blueberry in British Columbia, Canada. Canadian Journal of Plant Pathology 28: 250-262.

Zar, J .H. 1984. Biostatistical Analysis. Prentice-Hall, Englewood Cliffs, NJ.


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