Antennal detection of sex pheromone by female Pandemis limitata (Robinson) (Lepidoptera: Tortricidae) and its impact on their calling behaviour

Naomi C. DeLury, Gary J. R. Judd, Mark G. T. Gardiner


Previous observations lead us to believe that female Pandemis limitata (Robinson) (0 to 24 h old) are as attractive as their pheromone gland extract to males in clean air, but are more attractive in an environment permeated with their major pheromone component(Z)-11-tetradecenyl acetate. Therefore, in this study, we tested the hypothesis that females can detect and/or respond to their pheromone components. Using electroantennographic detection, we found female P. limitata able to perceive both of their known pheromone components, (Z)-11-tetradecenyl acetate and (Z)-9-tetradecenyl acetate. Female antennal response was found to be 46.3% weaker than that of males, under identical conditions, with male antennae producing significantly higher deflections to the higher pheromone doses tested and to the plant volatile,(E)-2-hexanal. Observations of females in clean air versus (Z)-11-tetradecenyl acetate-permeated air showed no significant differences with respect to onset time, frequency or duration of calling. Females moved significantly less often in a (Z)-11-tetradecenyl acetate-permeated portion of a flight tunnel than in the corresponding clean-air portion.


(Z)-11-tetradecenyl acetate; (Z)-9-tetradecenyl acetate; female electroantennography; flight tunnel; mating disruption; threelined leafroller; sprayable pheromone; microencapsulated pheromone; movement

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